Plasmid

Part:BBa_I713028:Design

Designed by: Zhenyu Shi   Group: iGEM07_Tsinghua   (2007-10-26)


pEB-C1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1078
    Illegal SpeI site found at 258
    Illegal PstI site found at 1038
    Illegal PstI site found at 2228
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 258
    Illegal PstI site found at 1038
    Illegal PstI site found at 2228
    Illegal NotI site found at 1059
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2015
    Illegal BamHI site found at 252
    Illegal BamHI site found at 1018
    Illegal XhoI site found at 1066
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1078
    Illegal SpeI site found at 258
    Illegal PstI site found at 1038
    Illegal PstI site found at 2228
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1078
    Illegal SpeI site found at 258
    Illegal PstI site found at 1038
    Illegal PstI site found at 2228
    Illegal NgoMIV site found at 1404
    Illegal NgoMIV site found at 2679
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3578
    Illegal SapI.rc site found at 2528
    Illegal SapI.rc site found at 2738


Design Notes

NcoI-SD series-C1 gene-BamHI


Source

Lambda DNA (NEB) pEASY-B

References